FIGURE

Fig. 1

ID

ZDB-FIG-130624-15

Publication

Wang et al., 2013 - Genetic Interaction between pku300 and fbn2b Controls Endocardial Cell Proliferation and Valve Development in Zebrafish

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Fig. 1

Endocardial and cardiac valve defects were found in sco^te382 mutant embryos. (A,B) Brightfield views of live wild-type (A) and sco^te382 mutant (B) embryos at 48hpf. Note pericardial edema and heart abnormality (black arrowhead), as well as abnormal vessels in the caudal trunk (red arrowhead). (C,D) Confocal images of Tg(myl7:eGFP) transgenic wild-type (C) and sco^te382 mutant (D) hearts at 48hpf. Note lack of constricted AVC and dilated heart in sco^te382 mutant (arrowhead). (E,F) Hematoxylin-eosin staining of wild-type (E) and sco^te382 mutant (F) hearts at 48hpf on transverse JB4 sections. Note lack of AVC constriction (arrowhead) in sco^te382 mutant heart. (G,H) Valve leaflets (arrowhead) were labeled and detected by the Tg(kdrl:eGFP) transgene in wild-type (G), but not in sco^te382 mutant (H) embryos at 6dpf on vibrotome sections across the heart. (I–N) Endocardial markers fli1 (I,J) and tie1 (K,L) were expressed in wild-type (I,K) and sco^te382 mutant (J,L) embryos; and Tg(kdrl:eGFP) labeled the endocardium in wild type (M) and sco^te382 mutant (N) embryos at 48hpf. Note that endocardial genes (fli1, tie1 and flk1) were reduced in the atrium of sco^te382 mutants (J,L,N). Arrows point to the AVC. a, atrium; v, ventricle.

Expression Data

Genes:	fli1 GFP tie1
Fish:	WT f1Tg la116Tg fbn2b^{te382a/te382a}; pku300^{te382b/te382b} fbn2b^{te382a/te382a}; pku300^{te382b/te382b}; f1Tg fbn2b^{te382a/te382a}; pku300^{te382b/te382b}; la116Tg
Anatomical Terms:	atrial endocardium atrial myocardium atrioventricular canal endocardium ventricular endocardium ventricular myocardium
Stage Range:	Long-pec to Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fbn2b^te382a/+; pku300^te382b/+; la116Tg fbn2b^{te382a/te382a}; pku300^{te382b/te382b} fbn2b^{te382a/te382a}; pku300^{te382b/te382b}; la116Tg
Observed In:	atrioventricular canal atrioventricular valve atrioventricular valve formation heart post-vent vasculature
Stage Range:	Long-pec to Day 6

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.