ZFIN ID: ZDB-FIG-130618-5
Grotek et al., 2013 - Notch signaling coordinates cellular proliferation with differentiation during zebrafish fin regeneration. Development (Cambridge, England)   140(7):1412-1423 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
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Anatomical Terms:
Stage: Adult

Fig. 1 Notch signaling is activated in the blastema during regenerative outgrowth. (A) EGFP fluorescence in her4.3:EGFP transgenics. By 96 hpa, EGFP signal is strongly intensified (exposure times: 0, 36 and 48 hpa for 7 seconds; 96 hpa for 0.5 seconds). (B) Longitudinal sections of her4.3:EGFP regenerates at 3 dpa stained for egfp. There is expression in the proximal but not the distal blastema, and weak expression in interray tissue. (C) her4.1 and her12 levels in 3 dpa regenerates measured by qRT-PCR, shown relative to the level in the distal-most stump segment at 50% fin length (0 hpa). (D) FACS scatterplot showing cell fractions of EGFP+ and EGFP- cells from dissociated 4 dpa her4.3:EGFP regenerates. (E) Endogenous her4.1 transcripts can be detected only by semi-quantitative PCR in the EGFP+ fraction of her4.3:EGFP regenerates. (F) her2, her9 and hey1 expression is downregulated in 3 dpa regenerates treated with DAPT for 6 hours. (G) her6 is expressed in the blastema at 3 dpa. (H) her4.3-driven egfp transcripts (red) and endogenous her6 (blue) are downregulated in regenerates treated with LY411575 6 hours after the start of treatment (hpt) (n=6/6 fins) and not detectable at 24 hpt (n=5/6). (I) her4.3-driven EGFP fluorescence is downregulated in 3 dpa regenerates treated with LY411575 for 24 hours, but not for 6 hours. (J) jag1b is expressed in the blastema at 3 dpa. (A-J) Arrowheads indicate amputation plane. Scale bars: whole mounts, 200 μm; sections, 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y83Tg physical alteration: anatomical structure Adult blastema IFL
her2 WT physical alteration: anatomical structure, chemical treatment: DAPT Adult regenerating fin RTPCR
her4.1 y83Tg physical alteration: anatomical structure Adult blastema RTPCR
her6 WT physical alteration: anatomical structure Adult blastema ISH
y83Tg physical alteration: anatomical structure, chemical treatment: pharmaceutical Adult blastema ISH
her9 WT physical alteration: anatomical structure, chemical treatment: DAPT Adult regenerating fin RTPCR
her12 y83Tg physical alteration: anatomical structure Adult blastema RTPCR
hey1 WT physical alteration: anatomical structure, chemical treatment: DAPT Adult regenerating fin RTPCR
jag1b WT physical alteration: anatomical structure Adult blastema ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab16-GFP IHC y83Tg physical alteration: anatomical structure Adult blastema
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development