ZFIN ID: ZDB-FIG-130510-8
Münch et al., 2013 - Notch regulates blastema proliferation and prevents differentiation during adult zebrafish fin regeneration. Development (Cambridge, England)   140(7):1402-1411 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Adult

Fig. 1

Notch signalling is activated during fin regeneration. (A) qPCR analysis of notch1b, lfng, her6 and her15 mRNA in regenerating fins at 3 dpa relative to expression in non-amputated fins (0 dpa). *P<0.05, **P<0.01. (B-F) Whole-mount in situ hybridization in 2 dpa fins for jag1b (B), notch1b (C), lfng (D), her6 (E) and her15 (F), showing Notch pathway activation within the blastema (arrowheads). (G) Scheme of a 3 dpa fin section, indicating the two regions within the regenerate: the blastema (b) and the differentiation zone (dz). Green indicates regenerating fin rays. (H-J) In situ hybridization on 3 dpa fin sections for jag1b (H), lfng (I) and her6 (J) reveals Notch pathway activation in the blastema (b, arrowhead), but weak or no gene expression within the dz (arrow). Scale bars: 100 μm in C,F; 10 μm in J. Broken lines mark amputation plane.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
her6 WT physical alteration: anatomical structure Adult blastema ISH
Adult regenerating fin ISH
her15.2 WT physical alteration: anatomical structure Adult blastema ISH
jag1b WT physical alteration: anatomical structure Adult blastema ISH
Adult regenerating fin ISH
lfng WT physical alteration: anatomical structure Adult blastema ISH
Adult regenerating fin ISH
notch1b WT physical alteration: anatomical structure Adult blastema ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development