FIGURE

Fig. 9

ID

ZDB-FIG-130426-30

Publication

Dooley et al., 2013 - On the embryonic origin of adult melanophores: the role of ErbB and Kit signalling in establishing melanophore stem cells in zebrafish

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Fig. 9

Abnormal NC migration and apoptosis in slk embryos. (A-B′) GFP-positive cells in double transgenic Tg(mitfa:gfp; nbt:DsRed) wild-type (A,A′) and slk mutant (B,B′) embryos at 48 hpf. In the wild-type embryo, GFP-positive NC cells remain at the position of the DRG and have a rounded morphology (white arrowhead in A,A2) whereas in slk mutants they are stretched along the nerves and appear to be migratory (white arrowheads in B,B2). A′, B′ show green channel only. (C,D) Magnification showing mitfa:gfp-labelled cells at the exit point of the spinal nerves at 48 hpf of wild-type (C) and slk mutant (D) embryos. The arrowheads point to DRGs. (E-F′) TUNEL staining in Tg(mitfa:gfp) embryos in wild-type (E,E′) and slk (F,F′) embryos at 20 hpf. E′,F′ show red channel only. (G-H′) Wild type (G) and slk morphant (H,H′) at 20 dpf (5.7 mm SL).

Expression Data

Genes:	DsRed GFP
Fish:	w47Tg; zf148Tg kitlga^{tc244b/tc244b}; w47Tg; zf148Tg
Anatomical Terms:	dorsal root ganglion glial cell melanoblast neuron spinal nerve
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-kitlga kitlga^{tc244b/tc244b}; w47Tg kitlga^{tc244b/tc244b}; w47Tg; zf148Tg
Knockdown Reagent:	MO1-kitlga
Observed In:	apoptotic process melanocyte trunk neural crest cell trunk neural crest cell migration whole organism
Stage Range:	20-25 somites to Days 14-20

Phenotype Detail

Acknowledgments

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