FIGURE

Fig. 2

ID
ZDB-FIG-130128-2
Publication
Torregroza et al., 2012 - Regulation of a Vascular Plexus by gata4 Is Mediated in Zebrafish through the Chemokine sdf1a
Other Figures
All Figure Page
Back to All Figure Page
Fig. 2

The vascular morphology of the CHT is disrupted in the gata4 morphant embryo.

Shown are representative embryos (n >50) derived from tg(gata1:dsred; fli1:egfp) double transgenic reporter fish. The green signal shows the vascular endothelium, while the red signal shows the circulation of blood cells, which are moving so quickly that individual cells are not seen. At 28 hpf (A,B) the blood circulates to the caudal end of the developing CHT (the caudal end is indicated by white arrows) in both control uninjected (A) and gata4 morphant (B) embryos. However, by 72 hpf (C,D), the vascular plexus is much more developed in the control (C) compared to the morphant (D). In addition, the blood circulation is restricted in the morphant, failing to move into the more caudal regions, as indicated in panels C and D by the white brackets that mark the distance from the caudal end of the CHT to the most caudal position of circulating cells (indicated by the white arrows). The block to plexus formation can be seen clearly at 48 hpf (E,F), when in the control embryos (E) the dorsal aorta and caudal vein are well separated to create a fenestrated space (this space is indicated by double-headed arrows), which is not seen in the morphant (F). MO = morphant. Views are lateral, anterior to the left.

Expression Data
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One