FIGURE

Fig. 4

ID

ZDB-FIG-121214-20

Publication

Stahlhut et al., 2012 - miR-1 and miR-206 regulate angiogenesis by modulating VegfA expression in zebrafish

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Fig. 4

Target protectors relieve miR-1/206-mediated repression of the vegfaa 32UTR. (A) Sequence of each target site, the cognate target protector (TP, violet) and miR-1. (B) Luciferase assay to test the action of vegfaa TP as described in Fig. 2. (C) Luciferase reporter assay to determine the ability of the TPs to prevent miR-1/206-mediated repression of the luciferase-vegfaa 3′UTR. Repression of the luciferase-vegfaa 3′UTR is relieved in the presence of vegfaa TPs (+TP). (D) Confocal analysis of GFP expression in the ISV of Tg(fli-nGFP)^y7 zebrafish embryos injected with control MO or vegf-TP^miR-1 at the one-cell stage. (E) Quantitation of the number of nuclei per ISV of control MO-, miR-1/206 MO- or TP-injected embryos. Note the increase in endothelial cells in the ISV of embryos injected with TP against the miR-1/206 target sites in the vegfaa 3′UTR. (C,E) Mean ± s.d.; Student′s t-test.

Expression Data

Gene:	EGFP
Fish:	y7Tg
Anatomical Terms:	dorsal aorta nucleus intersegmental vessel nucleus vascular endothelium nucleus
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	y7Tg + MO1-mir1-1,mir1-2 + MO1-mir206-1
Knockdown Reagents:	MO1-mir1-1,mir1-2 MO1-mir206-1
Observed In:	blood vessel endothelial cell intersegmental vessel
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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