FIGURE

Fig. 5

ID
ZDB-FIG-121214-15
Publication
Li et al., 2012 - SUMO1-activating enzyme subunit 1 is essential for the survival of hematopoietic stem/progenitor cells in zebrafish
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Fig. 5

Loss of Sae1 function impairs the sumoylation pathway. (A-D) WISH shows the hbae1 and lyz expression in 23 hpf wild-type fish and ubc9 morphants. Black arrows indicate the ICM. Blue arrows indicate the yolk sac. (E,F) WISH indicates the cmyb expression in 36 hpf wild-type fish and ubc9 morphants. Arrows indicate the AGM of zebrafish. (G-J) WISH shows the cmyb expression in 3-4 dpf wild-type fish and ubc9 morphants. Arrows indicate the CHT region. (K) Western blotting indicates the expression of free SUMO1s and the SUMO1 conjugates in 6 dpf siblings (left) and tangohkz5/hkz5 mutant embryos (right). (L) Western blotting shows the expression of free SUMO2/3 and the SUMO2/3 conjugates in 6 dpf siblings (left) and tangohkz5/hkz5 mutant embryos (right). (M) Quantification of the SUMO1 conjugates and the free SUMO1 relative to tubulin. (N) Quantification of the SUMO2/3 conjugates and the free SUMO2/3 relative to actin.

Expression Data
Genes:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms: (all 6)
Stage Range: 1-cell to Day 6

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 6

Phenotype Detail
Acknowledgments
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