Fig. S3
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- ZDB-FIG-121031-22
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- Khan et al., 2012 - Pou2, a class V POU-type transcription factor in zebrafish, regulates dorsoventral patterning and convergent extension movement at different blastula stages
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Expression of dorsoventral marker genes in embryos injected with mRNA for pou2-related genes. The expression of dorsoventral marker genes, shown on the left, was examined at the bud stage (A–J) or at 25 hpf (K–T) in embryos injected with mRNA for egfp (A, F, K, P), pou2 (B, G, L, Q), vp-pou2 (C, H, M, R), mouse Oct-3/4 (D, I, N, S), and en-pou2 (E, J, O, T). The numbers of embryos with abnormal expression patterns and total numbers of embryos examined are shown at the bottom of the respective panels. (A–J) The expression of neural markers in the dorsal neural plate became narrower in embryos overexpressing pou2, vp-pou2, and Oct-3/4, whereas it expanded ventrally in en-pou2-overexpressing embryos. In embryos overexpressing en-pou2 (E), hoxb1b expression was expanded ventrally, which can be seen in the flank region as marked by white arrows. (F-I) The intervals between parallel dashed lines are the same. (K–T) The expression of gata1a and tbx6 in the ICM was expanded by pou2, vp-pou2, and Oct-3/4, but downregulated by en-pou2. tbx6 expression in the tail bud was little affected. icm, intermediate cell mass; tb, tail bud. Scale bar, 200 μm. |
Reprinted from Mechanisms of Development, 129(9-12), Khan, A., Nakamoto, A., Okamoto, S., Tai, M., Nakayama, Y., Kobayashi, K., Kawamura, A., Takeda, H., and Yamasu, K., Pou2, a class V POU-type transcription factor in zebrafish, regulates dorsoventral patterning and convergent extension movement at different blastula stages, 219-235, Copyright (2012) with permission from Elsevier. Full text @ Mech. Dev.