Fig. 1
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- ZDB-FIG-121031-11
- Publication
- Khan et al., 2012 - Pou2, a class V POU-type transcription factor in zebrafish, regulates dorsoventral patterning and convergent extension movement at different blastula stages
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The strategy of the overexpression of pou2 and its related genes. (A) Structures of the proteins encoded by pou2, vp-pou2, en-pou2, and mouse Oct-3/4. SD, POU-specific domain; HD, POU homeodomain. (B) The structure of the Hsp-En-Pou2 gene (HEP). The PCR primers used to detect the HEP transgene by genotyping of embryos are shown by a pair of blue arrows. (C) Identification of the HEP-containing transgenic (Tg) fish by detecting the transgene sequence using PCR in offspring embryos followed by agarose gel electrophoresis (arrow). 1, Phage lambda DNA digested by HindIII, used as size markers; 2, PCR product obtained using the HEP construct as a template; 3, PCR product obtained by genotyping of offspring embryos from HEP Tg fish. (D) HEP Tg embryos were exposed to heat shock for 1 h (9 hpf) and examined 1 h later (bud stage) for the expression of pou2 by whole-mount in situ hybridization. When embryos were not exposed to high temperature (–heat shock), the endogenous pou2 expression alone was detected, whereas heat treatment induced pou2 expression throughout the embryo (+heat shock). Scale bar, 200 μm. |
Reprinted from Mechanisms of Development, 129(9-12), Khan, A., Nakamoto, A., Okamoto, S., Tai, M., Nakayama, Y., Kobayashi, K., Kawamura, A., Takeda, H., and Yamasu, K., Pou2, a class V POU-type transcription factor in zebrafish, regulates dorsoventral patterning and convergent extension movement at different blastula stages, 219-235, Copyright (2012) with permission from Elsevier. Full text @ Mech. Dev.