FIGURE

Fig. 4

ID

ZDB-FIG-120914-23

Publication

Keow et al., 2012 - Activity-based labeling of matrix metalloproteinases in living vertebrate embryos

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

HxBP can be UV-photocrosslinked in vivo, and detected in situ by confocal microscopy.

Composite confocal micrographs of 24 hpf zebrafish embryos injected anteriorly with 50 μM trifunctional HxBP probe, recovered, and exposed to increasing levels of UV irradiation reveals increasingly spatially structured patterns of fluorescence up to 72 mJ/cm². Structures exhibiting strong HxBP labeling in 24 hpf embryos include the retina (r) and lens (l), head mesenchyme (hm), hatching gland (hg), perichordal sheath (ps), isolated notochord cells in the elongating region of the notochord (n), maturing myotome boundaries (mb), mesenchymal tissues of the trunk and tail (m), and the basement membrane underlying the epithelium dorsal to the elongating tail (bm). Asterisks mark the point of injection.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One