FIGURE

Fig. 6

ID
ZDB-FIG-120831-14
Publication
Eom et al., 2012 - Melanophore Migration and Survival during Zebrafish Adult Pigment Stripe Development Require the Immunoglobulin Superfamily Adhesion Molecule Igsf11
Other Figures
All Figure Page
Back to All Figure Page
Fig. 6

Igsf11 promotes aggregation of K562 myeloid leukemia cells in vitro.

Cells were transfected with wild-type zebrafish igsf11 (A, B), S151P, seuratutr15e1 mutant igsf11 (C, D), T29P, seuratwp15e2 mutant igsf11 (E, F), or mock transfected (G, H). At the start of the experiment, the numbers of small cellular aggregates and total numbers of cells were similar (A, C, E, G). By 120 min, a relatively small number of aggregates containing numerous cells had formed in the cells transfected with wild-type igsf11 (arrowheads in B), though this was not the case in cells of other treatments (D, F, H). (I) Quantitation of the ratio of cellular aggregates to the total number of cells (meanĀ±SE) confirmed that cells were increasingly found in fewer, larger aggregates when transfected with wild-type igsf11. At 120 min, degrees of aggregation differed significantly among treatments overall (F3,48 = 19.8, P<0.0001). Post hoc means comparisons indicated that aggregation behavior in cells transfected with wild-type igsf11 (B in the figure) differed significantly from that of cells transfected with mutant igsf11 or controls (A in the figure; Tukey Kramer post hoc comparisons, P<0.01); aggregation of cells transfected with mutant forms of igsf11 did not differ significantly from one another or from mock transfected cells. Values shown are least squares means adjusted to control for variation after controlling for minor but significant variation among replicates (P<0.01).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.