FIGURE

Fig. 3

ID

ZDB-FIG-120718-22

Publication

Cibrián Uhalte et al., 2012 - In vivo conditions to identify prkci phosphorylation targets using the analog-sensitive kinase method in zebrafish

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Fig. 3

Mutant Prkci^I316A uses bulky N⁶-benzyl ATPγS.

Kinase reaction with Myelin Basic Protein (MBP) and ATPγS or N⁶-benzyl ATPγS (6-bn- ATPγS), followed by PNBM alkylation. In comparison, only mutant Prkci^I316A efficiently utilizes N⁶-benzyl ATPγS to thiophosphorylate MBP. Labeled MBP is detected by Western blot analysis with rabbit monoclonal anti-thiophosphoester antibody (α-thioP).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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