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Fig. 3
Mutant PrkciI316A uses bulky N6-benzyl ATPγS.
Kinase reaction with Myelin Basic Protein (MBP) and ATPγS or N6-benzyl ATPγS (6-bn- ATPγS), followed by PNBM alkylation. In comparison, only mutant PrkciI316A efficiently utilizes N6-benzyl ATPγS to thiophosphorylate MBP. Labeled MBP is detected by Western blot analysis with rabbit monoclonal anti-thiophosphoester antibody (α-thioP).
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