FIGURE

Fig. 5

ID

ZDB-FIG-120412-24

Publication

Veleri et al., 2012 - Knockdown of Bardet-Biedl Syndrome Gene BBS9/PTHB1 Leads to Cilia Defects

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Fig. 5

Knockdown of bbs9 affects cilia in Kupffer′s vesicle.

(A) Schematic view of Kupffer′s vesicle (KV) in zebrafish embryo at 12 hpf. A, P, D and V indicate anterior, posterior, dorsal and ventral sides, respectively. (B) Morphant injected with control-MO (0.3 ng). (C) Morphant injected with bbs9-spMO (0.3 ng). In the morphants, KV cilia were visualized by staining with both anti-α-tubulin and anti-γ-tubulin (green), between 10–13 hpf. In ‘B’, cilia are more in number and are longer (cf. white arrows) than in ‘C’. In B and C, upper panels show the nuclei visualized with DAPI. (D) and (E) show quantification of KV cilia number and length, respectively. The Y-axis represents the mean ± SEM. The X-axis represents the indicated category of morphants analyzed.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-bbs9
Knockdown Reagent:	MO2-bbs9
Observed In:	Kupffer's vesicle cilium
Stage:	5-9 somites

Phenotype Detail

Acknowledgments

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