ZFIN ID: ZDB-FIG-120216-30
Balczerski et al., 2012 - Analysis of Sphingosine-1-phosphate signaling mutants reveals endodermal requirements for the growth but not dorsoventral patterning of jaw skeletal precursors. Developmental Biology   361(2):230-241 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Prim-25

Fig. 4

Variable disorganization of Shh-expressing endoderm and fgf8a ectodermal expression in s1pr2 and spns2b1110 mutants. (A–D) In situ hybridizations at 36 hpf show two classes of shha expression in s1pr2 mutants. When viewed dorsally Class 1 mutants have a wider shha endodermal expression domain (green arrows) than Class 2 mutants, and in lateral views of the same embryos Class 1 but not Class 2 mutants display ectopic shha expression (arrowheads) anterior to the normal expression domains (arrows). (E–H) In situ hybridizations at 24 hpf also show two classes of shhb expression in s1pr2 mutants, with Class I mutants (n = 3/8) showing ectopic anterior expression and Class 2 mutants (n = 5/8) displaying posterior truncation of the normal endodermal expression domain (arrows). (I–L) Several examples of spns2b1110 Class I mutants show fragmented expression of fgf8a expression in the oral ectoderm (arrowheads). Scale bar = 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
fgf8a WT standard conditions Prim-25 oral region ectoderm ISH
spns2b1110/b1110 standard conditions Prim-25 oral region ectoderm ISH
shha WT standard conditions Prim-25 endoderm ISH
s1pr2m93/m93 standard conditions Prim-25 endoderm ISH
shhb WT standard conditions Prim-5 endoderm ISH
s1pr2m93/m93 standard conditions Prim-5 endoderm ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Reprinted from Developmental Biology, 361(2), Balczerski, B., Matsutani, M., Castillo, P., Osborne, N., Stainier, D.Y., and Crump, J.G., Analysis of Sphingosine-1-phosphate signaling mutants reveals endodermal requirements for the growth but not dorsoventral patterning of jaw skeletal precursors, 230-241, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.