FIGURE

Fig. 3

ID
ZDB-FIG-120216-29
Publication
Balczerski et al., 2012 - Analysis of Sphingosine-1-phosphate signaling mutants reveals endodermal requirements for the growth but not dorsoventral patterning of jaw skeletal precursors
Other Figures
All Figure Page
Back to All Figure Page
Fig. 3

Facial ectoderm gene expression in endoderm mutants. (A–T) In situ hybridizations at 36 hpf. In wild types, pitx2ca (A), shha (E), and fgf8a (I) are expressed in oral ectoderm and edn1 (M) and bmp4 (Q) in aboral ectoderm. pitx2ca expression in the oral ectoderm (arrowheads) is present but shifted anteriorly in sox32 (B, n = 6/6), s1pr2 (C, n = 11/11), and spns2b1110 (D, n = 4/4) embryos. The expression of shha in the oral ectoderm is lost in all mutants, whereas endoderm expression (arrows) is absent in sox32 mutants (F, n = 7/7), variably posteriorly displaced (G, n = 10/15) or disorganized (see 4, n = 3/15) in s1pr2 mutants, and variably posteriorly displaced (H, n = 6/7) or disorganized (not shown, n = 1/7) in spns2b1110 mutants. fgf8a expression is reduced in sox32 mutants (J, n = 6/6), reduced (K, n = 4/10) or disorganized (not shown, n = 4/10) in s1pr2 mutants, and reduced (not shown, n = 13/27) or disorganized (L, n = 14/27) in spns2b1110 mutants. edn1 expression in the first two arches (white lines) is present but disorganized in sox32 (N, n = 8/8), s1pr2 (O, n = 6/6), and spns2b1110 (P, n = 5/5) mutants. bmp4 expression is also disorganized in sox32 (R, n = 6/6), s1pr2 (S, n = 7/7), and spns2b1110 (T, n = 5/5) mutants. Outlines of the oral ectoderm and first two pouches are shown for wild types. Scale bar = 50 μm.

Expression Data
Genes:
Fish:
Anatomical Terms:
Stage: Prim-25

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage: Prim-25

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 361(2), Balczerski, B., Matsutani, M., Castillo, P., Osborne, N., Stainier, D.Y., and Crump, J.G., Analysis of Sphingosine-1-phosphate signaling mutants reveals endodermal requirements for the growth but not dorsoventral patterning of jaw skeletal precursors, 230-241, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.