FIGURE

Fig. 2

ID

ZDB-FIG-120206-35

Publication

Bedell et al., 2012 - The lineage-specific gene ponzr1 is essential for zebrafish pronephric and pharyngeal arch development

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Fig. 2

ponzr1 expression in the developing zebrafish kidney. (A) lhx1a mRNA expression in the intermediate mesoderm (arrows) at 8S. (B) ponzr1 mRNA expression in the intermediate mesoderm (arrows) at 8S. (C,D) pax2a mRNA expression at 12S (C) is comparable to ponzr1 (D). (E-K) ponzr1 expression is maintained throughout kidney development from intermediate mesoderm to the formation of the pronephric ducts and tubules. Lateral (E) and dorsal (F) views of ponzr1 show expression in the intermediate mesoderm (arrow) at 10S. ponzr1 expression in the pronephric ducts and tubules (arrows) at 13S (G), 15S (H), 18S (I), 21S (J) and 24 hpf (K). (L-O) At 48 hpf (L,M) and 72 hpf (N,O), ponzr1 expression is detected in the pharyngeal arches (open arrows), pronephric tubules (black arrowhead), ducts (black arrow) and glomeruli (open arrowhead). (P,Q) Double in situ hybridizations with pax2a (orange) and ponzr1 (purple) reveal overlapping expression of ponzr1 and pax2a during kidney development (arrows).

Expression Data

Genes:	lhx1a pax2a ponzr1
Fish:	WT
Anatomical Terms:	intermediate mesoderm pharyngeal arch pronephric duct pronephric glomerulus pronephric tubule pronephros
Stage Range:	5-9 somites to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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