FIGURE

Fig. 4

ID

ZDB-FIG-111128-82

Publication

Ben et al., 2011 - Targeted mutation of the talpid3 gene in zebrafish reveals its conserved requirement for ciliogenesis and Hedgehog signalling across the vertebrates

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Fig. 4

Disrupted Hh signalling in the somites and neural tube of Zebrafish MZta3 mutant embryos. (A-C) MZta3 mutant 24 hpf embryos display a characteristic curled body (A) and ectopic lens marked by the expression of Prox1 (arrowhead) (C). (D,E). Expanded domain of eng2a:GFP reporter gene expression in MZta3 (E) relative to control, zygotically rescued Mta3 heterozygotes (D). (F,G) Antibody staining of endogenous Eng and Prox1 proteins reveals that expansion of Eng is specific to the Prox1-negative fast-twitch muscle fibres; conversely, there is a reduction in the number of Eng expressing slow-twitch muscle pioneer fibres (arrowheads). (H,I) Expression of ptc2 in somites of Mta3 (H) and MZta3 (I) embryos showing expansion in latter. (J,K) Expression of ptc2 in somites of Mta3 (J) and MZta3 (K) embryos following incubation in cyclopamine. (L,M) Expression of nkx2.2 in the ventral neural tube (L) is lost in MZta3 mutants (M, arrowhead).

Expression Data

Genes:	EGFP nkx2.2a ptch2
Antibodies:	Ab1-eng Ab2-Prox1
Fish:	WT si:ch211-185a18.2^i264/+; si:ch211-185a18.2ⁱ²⁶⁵; si:ch211-185a18.2^i262/+ si:ch211-185a18.2^i264/+; si:ch211-185a18.2ⁱ²⁶⁵; si:ch211-185a18.2^i262/+; i233Tg si:ch211-185a18.2ⁱ²⁶⁵; si:ch211-185a18.2^i262/+ si:ch211-185a18.2ⁱ²⁶⁵; si:ch211-185a18.2^i262/+; i233Tg
Anatomical Terms:	fast muscle cell fast muscle cell nucleus muscle pioneer nucleus neural tube ventral region slow muscle cell slow muscle cell nucleus solid lens vesicle somite
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	si:ch211-185a18.2^i264/+; si:ch211-185a18.2ⁱ²⁶⁵; si:ch211-185a18.2^i262/+ si:ch211-185a18.2^i264/+; si:ch211-185a18.2ⁱ²⁶⁵; si:ch211-185a18.2^i262/+; i233Tg
Observed In:	lens muscle pioneer skeletal muscle cell differentiation smoothened signaling pathway trunk
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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