ZFIN ID: ZDB-FIG-111101-36
Deng et al., 2011 - Dual roles for Rac2 in neutrophil motility and active retention in zebrafish hematopoietic tissue. Developmental Cell   21(4):735-745 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 2

Rac2D57N-Expressing Zebrafish Larvae Are More Susceptible to Bacterial Infection

(A) Left, demonstration of zebrafish larvae otic infection. Phenol red tracking dye (pink) was used to visualize bacterial infection. Sudan black staining of neutrophil recruitment 2 hr post ear infection (hpi) in Rac2 (middle) and D57N larvae (right) larvae. Scale bar represents 50 μm.

(B) Neutrophils recruited to the ear 2 hpi were quantified. n = 41 (Rac2) and 39 (D57N). p < 0.001, two-tailed Mann-Whitney U test.

(C) Neutrophils recruited to the ear 1 hpi were quantified in control and Rac2 morphants. n = 36 (Ctrl MO) and 57 (Rac2 MO). p < 0.001, two-tailed Mann-Whitney U test.

(D) Survival of larvae challenged with <5 × 104 cfu of PAK (pMKB1::mCherry). n = 16 (Rac2) and 18 (D57N).

(E) Recruitment of leukocytes to otic infection in Rac2 and D57N larvae. Zebrafish larvae from Tg(mpx:dendra2) crossed with Rac2 or D57N lines were infected with PAK (<104 cfu) into the left ear. Total leukocyte populations were immunostained with L-Plastin antibody. Neutrophils were visualized by Dendra2 green fluorescence. The right ear of the same fish was shown as control. Images are representative for at least 15 individual fish. Scale bar represents 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Dendra2 uwm4Tg; zf306Tg bacterial treatment by injection: Pseudomonas aeruginosa Protruding-mouth neutrophil IFL
uwm4Tg; zf307Tg bacterial treatment by injection: Pseudomonas aeruginosa Protruding-mouth neutrophil IFL
lcp1 uwm4Tg; zf306Tg bacterial treatment by injection: Pseudomonas aeruginosa Protruding-mouth leukocyte IHC
uwm4Tg; zf307Tg bacterial treatment by injection: Pseudomonas aeruginosa Protruding-mouth leukocyte IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-rac2 bacterial treatment by injection: Pseudomonas aeruginosa Protruding-mouth defense response to Gram-negative bacterium disrupted, abnormal
Protruding-mouth neutrophil chemotaxis disrupted, abnormal
Protruding-mouth neutrophil mediated immunity disrupted, abnormal
uwm4Tg; zf307Tg bacterial treatment by injection: Pseudomonas aeruginosa Protruding-mouth defense response to Gram-negative bacterium disrupted, abnormal
Protruding-mouth neutrophil mediated immunity disrupted, abnormal
Protruding-mouth neutrophil spatial pattern, abnormal
zf307Tg (AB) bacterial treatment by injection: Pseudomonas aeruginosa Protruding-mouth defense response to Gram-negative bacterium disrupted, abnormal
Protruding-mouth neutrophil chemotaxis disrupted, abnormal
Protruding-mouth neutrophil mediated immunity disrupted, abnormal
Protruding-mouth whole organism viability, abnormal
Acknowledgments:
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Reprinted from Developmental Cell, 21(4), Deng, Q., Yoo, S.K., Cavnar, P.J., Green, J.M., and Huttenlocher, A., Dual roles for Rac2 in neutrophil motility and active retention in zebrafish hematopoietic tissue, 735-745, Copyright (2011) with permission from Elsevier. Full text @ Dev. Cell