PUBLICATION

Dual roles for Rac2 in neutrophil motility and active retention in zebrafish hematopoietic tissue

Authors
Deng, Q., Yoo, S.K., Cavnar, P.J., Green, J.M., and Huttenlocher, A.
ID
ZDB-PUB-111027-48
Date
2011
Source
Developmental Cell   21(4): 735-745 (Journal)
Registered Authors
Huttenlocher, Anna
Keywords
none
MeSH Terms
  • Actins/metabolism
  • Agammaglobulinemia/complications
  • Animals
  • Animals, Genetically Modified
  • Bacteria
  • Bacterial Infections/complications
  • Blotting, Western
  • Bone Marrow/metabolism
  • Cell Movement/physiology*
  • Fluorescent Antibody Technique
  • HL-60 Cells
  • Hematopoietic System/physiology*
  • Humans
  • Immunologic Deficiency Syndromes/complications
  • Larva/metabolism
  • Larva/microbiology
  • Mutation/genetics
  • Neutrophils/cytology*
  • Neutrophils/metabolism
  • RNA, Messenger/genetics
  • Receptors, CXCR4/genetics
  • Receptors, CXCR4/metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction
  • Warts/complications
  • Zebrafish/genetics*
  • Zebrafish/growth & development
  • Zebrafish/metabolism
  • rac GTP-Binding Proteins/physiology*
PubMed
22014524 Full text @ Dev. Cell
Abstract
Neutrophil homeostasis is essential for host defense. Here we identify dual roles for Rac2 during neutrophil homeostasis using a zebrafish model of primary immune deficiency induced by the human inhibitory Rac2D57N mutation in neutrophils. Noninvasive live imaging of Rac2 morphants or Rac2D57N zebrafish larvae demonstrates an essential role for Rac2 in regulating 3D motility and the polarization of F-actin dynamics and PI(3)K signaling in vivo. Tracking of photolabeled Rac2-deficient neutrophils from hematopoietic tissue also shows increased mobilization into the circulation, indicating that neutrophil mobilization does not require traditionally defined cell motility. Moreover, excessive neutrophil retention in hematopoietic tissue resulting from a constitutively active CXCR4 mutation in zebrafish warts, hypogammaglobulinemia, infections, and myelokathexis (WHIM) syndrome is partially rescued by the inhibitory Rac2 mutation. These findings reveal that Rac2 signaling is necessary for both neutrophil 3D motility and CXCR4-mediated neutrophil retention in hematopoietic tissue, thereby limiting neutrophil mobilization, a critical first step in the innate immune response.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes