FIGURE

Fig. 4

ID
ZDB-FIG-111028-6
Publication
Hale et al., 2011 - Netrin Signaling Breaks the Equivalence between Two Identified Zebrafish Motoneurons Revealing a New Role of Intermediate Targets
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Fig. 4

dscam mRNA is expressed in CaPs and VaPs and wildtype transcripts were knocked down with SBMOs.

A. A projected confocal stack of dscam mRNA (red) and isl2a mRNA (a CaP/VaP motoneuron marker [41]) expression (green), reveals that at 24 hpf dscam is expressed in both CaP and VaP (*; CaP and VaP pair to left, single CaP to right) as well as other cells that are likely motoneurons. B. RT-PCR results confirming uninjected embryos have wildtype dscam mRNA (395 bp band) while MO-injected embryos have increased dscam pre-mRNA (490 bp band). Starred lanes show dscam transcripts are absent after injecting 8.4 ng of dscam SBMO. Scale bar = 20 μm.

Expression Data
Gene:
Fish:
Anatomical Terms:
Stage: Prim-5

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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