ZFIN ID: ZDB-FIG-111025-14
Perlin et al., 2011 - Neuronal Neuregulin 1 type III directs Schwann cell migration. Development (Cambridge, England)   138(21):4639-4648 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 6

Overexpression of hNRG1 type III in all neurons leads to the ectopic migration of Schwann cells into the spinal cord. (A-K2) Images from in vivo time-lapse movies. Schwann cells are marked by the Foxd3:GFP transgene (green) and oligodendrocytes and motoneurons are marked by Olig2:dsRed (red). Numbers at top right denote time (minutes) elapsed since the start of the movie. (A-C) Schwann cells (carets) along motor nerves undergo little movement in the control embryo; asterisks mark motor nerves on the contralateral side. (D-I) Embryos overexpressing hNRG1 type III in all neurons. The open arrowhead follows a Schwann cell that migrates up a motor nerve and into the spinal cord; the white arrowhead follows a Schwann cell that divides and produces one daughter that moves into the spinal cord; and blue and purple asterisks follow Schwann cells already present in the spinal cord as they migrate along spinal axons. (J,K) Lateral views of single scans from in vivo time-lapse movies of control (J) and transgenic (K) embryos at 125 minutes. (J2,K2) Orthogonal view of the yz plane indicated by the vertical red lines in J and K. The open arrowhead marks the Schwann cell that is tracked by the open arrowhead in D-I. (L,M) Excess Schwann cells (arrows) are present along motor nerves of embryos overexpressing hNRG1 type III (M) as compared with control embryo (L) at 2 dpf. Anterior is to the left, dorsal is up. Comparable regions over the yolk extension were imaged. Embryos were genotyped after imaging. Scale bars: 50 μm in A-I,L-M; 20 μm in J,K.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 s1101tEt; st85Tg; vu19Tg; zf15Tg standard conditions Long-pec oligodendrocyte IHC
Long-pec spinal cord motor neuron IHC
Long-pec spinal cord myelinating Schwann cell IHC
vu19Tg; zf15Tg standard conditions Long-pec oligodendrocyte IHC
Long-pec spinal cord motor neuron IHC
GFP s1101tEt; st85Tg; vu19Tg; zf15Tg standard conditions Long-pec myelinating Schwann cell IHC
vu19Tg; zf15Tg standard conditions Long-pec myelinating Schwann cell IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s1101tEt; st85Tg; vu19Tg; zf15Tg standard conditions Long-pec myelinating Schwann cell located in spinal cord, abnormal
Long-pec Schwann cell migration decreased process quality, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development