Combinatorial knockdown of cBAF components does not prevent formation of endoderm and cranial/vascular mesoderm. (A-D) RNA in situ hybridization for expression of the endoderm-specific markers sox17 and fkd2 (foxa3) in triple gata5/smarcd3b/tbx5 morphants (sub-optimal doses of each as in Fig. 1) and control (wild-type) embryos. Embryos are at shield stage (6 hpf) lateral views with anterior towards the top of images (A,B) or at 24 hpf lateral views with anterior to left (C,D). (E,F) Cranial mesoderm of the jaw (marked with a tcf21:GVEcR;UAS:EGFP capsulin transgene) differentiates normally in triple gata5/smarcd3b/tbx5 morphants, but the show dismorphic jaw musculature. Transgene expression in the heart (arrowhead) is absent in morphants. (G.H) Vascular endothelial cell specification (as shown by a kdrl:EGFP transgene) is not affected in triple gata5/smarcd3b/tbx5 morphants. Boxed areas are shown at higher magnification in inset images, showing loss of kdrl:EGFP signal/endocardium in the cardiac region of morphant embryos. Lateral views of 48 hpf embryos are shown.
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