FIGURE

Fig. 3

ID

ZDB-FIG-110720-7

Publication

Anderson et al., 2011 - The L6 domain tetraspanin Tm4sf4 regulates endocrine pancreas differentiation and directed cell migration

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Fig. 3

The tm4sf4 gene and regulation by nkx2.2a is conserved in zebrafish. (A) CLUSTAL W species alignment between human, mouse and zebrafish Tm4sf4. Identical (^*), highly conserved (:) and weakly conserved (.) amino acids are indicated. Small/hydrophobic residues (red), acidic residues (blue), basic residues (magenta), hydroxyl/amine/basic residues (green) are shown. (B,C) Temporal qRT-PCR tm4sf4 mRNA analysis of early (B) and late (C) stage zebrafish embryos. (D-F) tm4sf4 in situ hybridization on wild-type (D,E) and nkx2.2a morphant (F) embryos. In, intestine; L, liver; P, pancreas. Images taken at 20× magnification. (G-I) qRT-PCR comparing uninjected (blue) and nkx2.2a morphant (red) embryo expression of tm4sf4 (G), insulin (H) and ghrelin (I). Error bars represent s.e.m.

Expression Data

Genes:	ghrl ins tm4sf4
Fish:	WT WT + MO3-nkx2.2a
Knockdown Reagent:	MO3-nkx2.2a
Anatomical Terms:	intestine liver pancreas pancreatic bud whole organism
Stage Range:	Dome to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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