FIGURE

Fig. 3

ID

ZDB-FIG-110622-49

Publication

Anastasaki et al., 2011 - Dhx34 and Nbas function in the NMD pathway and are required for embryonic development in zebrafish

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Fig. 3

Dhx34 is required for proper embryonic development in zebrafish. (A) Translation block and splice site MOs against zebrafish Dhx34 cause a distinctive range of developmental phenotypes at 24hpf characterized by shortening of the anteroposterior axis, somite malformation and significant reduction in head size. Morphants injected with the mismatch control MOs are indistinguishable from uninjected wild-type embryos. (B) Co-injection of Dhx34 translation block MO and human full-length DHX34 mRNA rescued the morphant phenotype in 73.8% of the injected embryos at 24 hpf. Expression of the mRNA alone has no overt effect on development. The numbers beside each panel indicate the percentage of embryos with the observed phenotype. Numbers in brackets correspond to the total of analysed embryos.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-dhx34 WT + MO2-dhx34
Knockdown Reagents:	MO1-dhx34 MO2-dhx34
Observed In:	embryo development head somite whole organism anterior-posterior axis
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.