FIGURE

Fig. S4

ID
ZDB-FIG-110516-25
Publication
Klein et al., 2011 - Neuron navigator 3a regulates liver organogenesis during zebrafish embryogenesis
Other Figures
All Figure Page
Back to All Figure Page
Fig. S4

Nav3a overexpression induces ectopic hepatic gene expression. (A) Cp is expressed in the liver bud of control morpholino-injected embryos at 48 hpf. Nav3a morphants show reduced cp expression in 60% of the analyzed embryos. In each group n=30 embryos were analyzed. (B) In the gain-of-function experiments pDestTol2-sox17-nav3aATGmut-mcherry plasmid was injected during single cell stages. Nav3a overexpression induced ectopic endodermal budding and dislocated endodermal cells in the foregut region. Ectopic budding outside of the foregut occurred in the trunk and the yolk sac (left panel). The nav3a positive buds expressed the hepatoblast marker hhex (arrowheads, right panel) (n=20). (C) Nav3a overexpression under control of the endodermal sox17 promoter induces regions of extra endodermal cp-expressing cell clusters. In each group n=50 embryos were analyzed. Ectopic buds were Nav3a-mCherry positive and in 90% of cases we observed cp expression in the ectopic bud (n=20). (D) The vascular marker flt4 (VEGF receptor-3) is not expressed in the ectopic buds (n=5).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development