ZFIN Figure: Padanad et al., 2011, Fig. 2

ZFIN ID: ZDB-FIG-110407-2

ZFIN ID: ZDB-FIG-110407-2

Padanad et al., 2011 - Pax2/8 proteins coordinate sequential induction of otic and epibranchial placodes through differential regulation of foxi1, sox3 and fgf24. Developmental Biology 351(1):90-98 Full text @ Dev. Biol.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	pax2a phox2a
Fish:	AB AB + MO3-sox3 AB + MO6-pax8 + MO7-pax8 AB + MO3-sox3 + MO6-pax8 + MO7-pax8
Knockdown Reagents:	MO3-sox3 MO6-pax8 MO7-pax8
Anatomical Terms:	epibranchial field epibranchial ganglion otic placode
Stage Range:	5-9 somites to Prim-15

PHENOTYPE:

Fish:	AB + MO3-sox3 AB + MO6-pax8 + MO7-pax8 AB + MO3-sox3 + MO6-pax8 + MO7-pax8
Knockdown Reagents:	MO3-sox3 MO6-pax8 MO7-pax8
Observed In:	epibranchial ganglion facial ganglion glossopharyngeal ganglion otic vesicle vagal ganglion 1 vagal ganglion 2 whole organism
Stage:	Prim-15

Fig. 2 pax8 and sox3 interact in otic and epibranchial induction. (A–D) pax2a expression in the otic/epibranchial domain at 12 hpf in a control embryo (A), sox3 morphant (B), pax8 morphant (C) and sox3–pax8 double morphant (D). Numbers indicate normalized values for the mean ± standard deviation of the area of the pax2a expression domain (n = 10 specimens for each background). Area was calculated by outlining otic–epibranchial domains in Photoshop and measuring the number of pixels within. Differences between the morphants and the control were highly significant (p<.0005) as determined by t-tests. (E–H) Otic vesicles at 30 hpf in a live control embryo (E), sox3 morphant (F), pax8 morphant (G) and sox3–pax8 double morphant (H). (I–L) Expression of phox2a at 30 hpf in a control embryo (I), sox3 morphant (J), pax8 morphant (K) and sox3–pax8 double morphant (L). Positions of the facial ganglion (f) glossopharyngeal ganglion (g), and vagal ganglia (v₁ and v₂) are indicated. All images show lateral views with anterior to the left.

Gene Expression Details

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay
pax2a	AB	control	5-9 somites		epibranchial field	ISH
			5-9 somites		otic placode	ISH
	AB + MO3-sox3	standard conditions	5-9 somites		epibranchial field	ISH
			5-9 somites		otic placode	ISH
	AB + MO3-sox3 + MO6-pax8 + MO7-pax8	standard conditions	5-9 somites		epibranchial field	ISH
			5-9 somites		otic placode	ISH
	AB + MO6-pax8 + MO7-pax8	standard conditions	5-9 somites		epibranchial field	ISH
			5-9 somites		otic placode	ISH
phox2a	AB	control	Prim-15		epibranchial ganglion	ISH
	AB + MO3-sox3	standard conditions	Prim-15		epibranchial ganglion	ISH
	AB + MO3-sox3 + MO6-pax8 + MO7-pax8	standard conditions	Prim-15	Not Detected	epibranchial ganglion	ISH
	AB + MO6-pax8 + MO7-pax8	standard conditions	Prim-15		epibranchial ganglion	ISH

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
AB + MO3-sox3	standard conditions	Prim-15	epibranchial ganglion decreased amount, abnormal
AB + MO3-sox3 + MO6-pax8 + MO7-pax8	standard conditions	Prim-15	otic vesicle decreased size, abnormal
		Prim-15	whole organism lacks all parts of type facial ganglion, abnormal
		Prim-15	whole organism lacks all parts of type glossopharyngeal ganglion, abnormal
		Prim-15	whole organism lacks all parts of type vagal ganglion 1, abnormal
		Prim-15	whole organism lacks all parts of type vagal ganglion 2, abnormal
AB + MO6-pax8 + MO7-pax8	standard conditions	Prim-15	otic vesicle decreased size, abnormal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Reprinted from Developmental Biology, 351(1), Padanad, M.S., and Riley, B.B., Pax2/8 proteins coordinate sequential induction of otic and epibranchial placodes through differential regulation of foxi1, sox3 and fgf24, 90-98, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.

Acknowledgments:

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 351(1), Padanad, M.S., and Riley, B.B., Pax2/8 proteins coordinate sequential induction of otic and epibranchial placodes through differential regulation of foxi1, sox3 and fgf24, 90-98, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.