ZFIN ID: ZDB-FIG-101123-50
Gamba et al., 2010 - Estrogen receptor ESR1 controls cell migration by repressing chemokine receptor CXCR4 in the zebrafish posterior lateral line system. Proceedings of the National Academy of Sciences of the United States of America   107(14):6358-6363 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-15
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-15

Fig. 3

Patterns of gene expression in the PLL primordium. (A and B) Expression of cxcr4b and cxcr7b in 32-hpf control embryos. (C and D) Expression of cxcr4b in conditions of esr1 loss-of-function (C) and gain-of-function (D). The change in expression between A and C was observed in 75% of the injected embryos, respectively, the change between A and D in 30% of the injected embryos. (E and F) Expression of lef1 and cxcr7b in esr1 loss-of-function conditions. (G and H) Expression of cxcr7b (blue) and of gfp (brown) in a 32-hpf control cldnb:gfp embryo (G), in an sdf1-MO embryo (H; an extreme case of cxcr7b domain expansion is shown in I). (J–L) Rescue of cxcr7b expression in esr1-MO embryos that are also sdf1-MO (J), mutant for ody (K), or morphant for cxcr4b (L; note the nerve, arrowhead, extending from the ganglion, asterisk at the left of the figure, under the stalled primordium). (M and N) Expression of cxcr4b in 32-hpf sdf1-MO injected (M) or ody - (N) homozygous embryos; the photograph in N was taken under Nomarski optics to illustrate the extent of the primordium. (O and P) Expression of esr1 in a 32-hpf migrating primordium as revealed by fluorescent in situ hybridization using a rhodamine-coupled antidioxygenin antibody (O) and coexpression of esr1 and cldnb:gfp in the same embryo (P; confocal imaging). (A–F, K, and M) are bright-field pictures without Nomarski optics, to facilitate the detection of expression patterns. The primordia are outlined by dotted lines, based on images taken under Nomarski optics as illustrated in N. Double-headed arrows indicate the extent of the region where cxcr7b expression is not detectable.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ackr3b WT standard conditions Prim-15 posterior lateral line primordium ISH
WT + MO1-esr1 standard conditions Prim-15 Not Detected posterior lateral line primordium ISH
cxcr4bt26035/t26035 + MO1-esr1 standard conditions Prim-15 posterior lateral line primordium ISH
zf106Tg standard conditions Prim-15 posterior lateral line primordium ISH
zf106Tg + MO1-esr1 + MO2-cxcl12a standard conditions Prim-15 posterior lateral line primordium ISH
zf106Tg + MO1-esr1 + MO7-cxcr4b standard conditions Prim-15 posterior lateral line primordium ISH
zf106Tg + MO2-cxcl12a standard conditions Prim-15 posterior lateral line primordium ISH
cxcr4b WT standard conditions Prim-15 posterior lateral line primordium ISH
WT + MO1-esr1 standard conditions Prim-15 posterior lateral line primordium ISH
WT + MO2-cxcl12a standard conditions Prim-15 posterior lateral line primordium ISH
cxcr4bt26035/t26035 standard conditions Prim-15 posterior lateral line primordium ISH
EGFP zf106Tg standard conditions Prim-15 posterior lateral line primordium IHC
zf106Tg + MO1-esr1 + MO2-cxcl12a standard conditions Prim-15 posterior lateral line primordium IHC
zf106Tg + MO1-esr1 + MO7-cxcr4b standard conditions Prim-15 posterior lateral line primordium IHC
zf106Tg + MO2-cxcl12a standard conditions Prim-15 posterior lateral line primordium IHC
esr1 zf106Tg standard conditions Prim-15 posterior lateral line primordium ISH
lef1 WT + MO1-esr1 standard conditions Prim-15 posterior lateral line primordium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-esr1 standard conditions Prim-15 posterior lateral line neuromast primordium migration process quality, abnormal
Prim-15 posterior lateral line primordium physical object quality, abnormal
WT + MO2-cxcl12a standard conditions Prim-15 posterior lateral line neuromast primordium migration process quality, abnormal
Prim-15 posterior lateral line primordium physical object quality, abnormal
cxcr4bt26035/t26035 standard conditions Prim-15 posterior lateral line neuromast primordium migration process quality, abnormal
Prim-15 posterior lateral line primordium physical object quality, abnormal
zf106Tg + MO1-esr1 + MO2-cxcl12a standard conditions Prim-15 posterior lateral line neuromast primordium migration process quality, abnormal
Prim-15 posterior lateral line primordium physical object quality, abnormal
zf106Tg + MO1-esr1 + MO7-cxcr4b standard conditions Prim-15 posterior lateral line neuromast primordium migration process quality, abnormal
Prim-15 posterior lateral line primordium physical object quality, abnormal
zf106Tg + MO2-cxcl12a standard conditions Prim-15 posterior lateral line neuromast primordium migration process quality, abnormal
Prim-15 posterior lateral line primordium physical object quality, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA