FIGURE

Fig. 7

ID
ZDB-FIG-101108-47
Publication
Poulton et al., 2010 - A novel role for Glucocorticoid-Induced TNF Receptor Ligand (Gitrl) in early embryonic zebrafish development
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Fig. 7

Gitrl and the Stat3-Liv1-Cdh1 axis. (A) Transcription of liv1 and cdh1 were assessed by real-time PCR at various time points during gastrulation and segmentation in embryos injected with 10 ng of either control1 or zGL1. Values were normalised to ef1α and levels in zGL1 injected embryos expressed relative to levels detected in control-injected embryos using the ΔΔCt method (ABI Prism 7700 Sequence Detection System, user bulletin no. 2). Mean values ± SD of three independent experiments are shown. Embryos injected with either (B,F) 10 ng of control 1 + 2.5 ng of cdh1 MO control (revcdh1), (C,G) 10 ng of zGL1 + 2.5 ng revcdh1, (D,H) 10 ng of zGL1 + 2.5 ng of cdh1 MO (cdh1) or (E,I) 10 ng of control 1 + 2.5 ng of cdh1 were observed at (B-E) 6 and (F-I) 8 hpf. Embryos treated with control MOs only were indistinguishable from wild-type embryos. Those treated with the zGL1 plus revcdh1 showed the characteristics of gitrl morphants previously described, and embryos injected with cdh1 MO plus control1 resembled the avalanche mutant (Kane et al., 1996). Surprisingly, embryos co-injected with both gitrl and cdh1 MOs had a novel phenotype characterized by arrest during the blastula period and a complete failure to enter epiboly. The phenotype shown in (D) and (H) persisted until the embryos died at approximately 15 hpf.

Expression Data
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: 30%-epiboly to Prim-5

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Shield to 14-19 somites

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Dev. Biol.