FIGURE

Fig. 4

ID

ZDB-FIG-100809-27

Publication

Xie et al., 2010 - A novel transgenic zebrafish model for blood-brain and blood-retinal barrier development

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Claudin-5a and 5b expression in hyaloid vasculature. (A) The 3′ ends of three zebrafish claudin genes have significant homology with the C-terminal of mouse claudin-5. Claudin-5a (zgc 85723; GenBank: NM_213274) and claudin-5b (zgc 103419; GenBank: NM_001006044) are mostly homologous to claudin-5a of Fugu rubripes, with 82% identical (plus 8% similar) and 75% identical (plus 14% similar) amino acid sequences, respectively. The zebrafish claudin-h (GenBank: NM_131767) is mostly homologous to claudin-3a of Fugu. (B-E′) Whole mount in situ hybridization of claudin-5a expression. (B) Claudin-5a is expressed in the CNS (midbrain, hindbrain, ventricular zone, and epiphysis) at 1 dpf. (C-E & C′-E′) From 1.5 to 2.5 dpf, claudin-5mRNA is detected in the hyaloid vasculature (black arrows), hyaloid artery (white arrows) and the cornea (arrow heads). (F-H) Claudin-5b is expressed in the entire vascular system at 1 dpf (arrow and arrowheads in F), and is confined to the blood vessels of the brain (arrows in G) and cardiovascular system (arrowhead in G) at 2 dpf. Similar to claudin-5a, expression of claudin-5b in the hyaloid vasculature (black arrow in H) and hyaloid artery (white arrow in H) is seen at 1.5 dpf and lasts till 3 dpf. B-E, dorsal view; C′-E′, side view; F-H, side view.

Expression Data

Genes:	cldn5a cldn5b
Fish:	WT
Anatomical Terms:	brain vasculature cardiovascular system cornea epiphysis hindbrain hyaloid artery hyaloid vein midbrain vasculature ventricular zone
Stage Range:	Prim-5 to Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.