FIGURE

Fig. 6

ID

ZDB-FIG-100809-29

Publication

Xie et al., 2010 - A novel transgenic zebrafish model for blood-brain and blood-retinal barrier development

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Fig. 6

Bradykinin mediated disruption of zebrafish BRB (A, A′ & B). The l-fabp:DBP-EGFP;flk1:mCherry double transgenic larvae were treated with 8 to 100 μM BK from 5 to 9 dpf. (A) DBP-EGFP (green) in hyaloid vessels of control (left panel), leaky hyaloid vessels (middle and right panel); (A′) endothelial lining of blood vessels, mCherry (red). (B) At 9 dpf, the larvae were scored for the presence of leaky hyaloid vessels. Both Fisher′s test and chi-square test indicate that the BK treatment results in significantly increased numbers of larvae showing GFP leakage compared with those treated with control buffer (P < 0.001). (C) Claudin-5 expression (red) was evaluated by whole mount immunohistochemistry in Tg(flk1:EGFP) larvae exposed to 100 μM BK and scored as strong expression (left panel), detectable (middle panel) and undetectable (right panel); (C′) endothelial lining of blood vessels, flk1:EGFP (green). (D) Approximately 47 BK treated and 46 untreated flk1:EGFP larvae were evaluated for claudin-5 expression in the hyaloid vasculature.

Expression Data

Genes:	EGFP mCherry
Antibody:	Ab1-cldn5
Fish:	s843Tg lri500Tg; s896Tg
Condition:	chemical treatment: bradykinin
Anatomical Terms:	blood blood vessel endothelial cell hyaloid vessel
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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