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Fig. S2

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ZDB-FIG-100628-40

Publication

Vaccari et al., 2010 - prep1.2 and aldh1a2 participate to a positive loop required for branchial arches development in zebrafish

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Fig. S2

Control experiments of Prep1.2 morpholinos. A: An embryo injected with a 6 bases mismatched prep1.2 morpholino develops two posterior streams of NCC (arrowheads) as evidenced by dlx2a expression pattern. B, C: loss of dlx2a expression in the posterior stream of NCC in embryos injected with MO2-prep1.2 is rescued when a prep1.2 mRNA lacking the morpholino target sequence is co-injected. D: An embryo injected with a 6 bases mismatched prep1.2 morpholino develops normal splanchnocranium. E, F: Rescue of defects in the posterior branchial arches of MO2-prep1.2 morphants by coinjection of a prep1.2 mRNA lacking the morpholino target sequence. G–I: Acridine orange staining in living embryos injected with prep1.2 morpholinos does reveal few p53-independent (G, n = 23) or p53-dependent (H, n = 27) cell death.

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

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Reprinted from Developmental Biology, 343(1-2), Vaccari, E., Deflorian, G., Bernardi, E., Pauls, S., Tiso, N., Bortolussi, M., and Argenton, F., prep1.2 and aldh1a2 participate to a positive loop required for branchial arches development in zebrafish, 94-103, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.