PUBLICATION

prep1.2 and aldh1a2 participate to a positive loop required for branchial arches development in zebrafish

Authors
Vaccari, E., Deflorian, G., Bernardi, E., Pauls, S., Tiso, N., Bortolussi, M., and Argenton, F.
ID
ZDB-PUB-100504-7
Date
2010
Source
Developmental Biology   343(1-2): 94-103 (Journal)
Registered Authors
Argenton, Francesco, Bortolussi, Marino, Deflorian, Gianluca, Pauls, Stefan, Tiso, Natascia, Vaccari, Enrico
Keywords
Vertebrate, Zebrafish, Endoderm, Segmentation, Prep, Retinoic acid, Pharyngeal arches
MeSH Terms
  • Embryo, Nonmammalian/metabolism
  • Retinal Dehydrogenase/genetics*
  • Retinal Dehydrogenase/metabolism
  • Animals
  • Body Patterning
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Zebrafish/embryology*
  • Zebrafish/metabolism
  • Transcription Factors/genetics*
  • Transcription Factors/metabolism
  • Branchial Region/embryology*
  • Gene Expression Regulation, Developmental
(all 13)
PubMed
20423710 Full text @ Dev. Biol.
Abstract
Segmentation is a key step in embryonic development. Acting in all germ layers, it is responsible for the generation of antero-posterior asymmetries. Hox genes, with their diverse expression in individual segments, are fundamental players in the determination of different segmental fates. In vertebrates, Hox gene products gain specificity for DNA sequences by interacting with Pbx, Prep and Meis homeodomain transcription factors. In this work we cloned and analysed prep1.2 in zebrafish. In-situ hybridization experiments show that prep1.2 is maternally and ubiquitously expressed up to early somitogenesis when its expression pattern becomes more restricted to the head and trunk mesenchyme. Experiments of loss-of-function with prep1.2 morpholinos change the shape of the hyoid and third pharyngeal cartilages while arches 4-7 and pectoral fins are absent, a phenotype strikingly similar to that caused by loss of Retinoic Acid (RA). In fact, we show that prep1.2 is positively regulated by RA and required for the normal expression of aldh1a2 at later stages, particularly in tissues involved in the development of the branchial arches and pectoral fins. Thus, prep1.2 and aldh1a2 are members of an indirect positive feedback loop required for pharyngeal endoderm and posterior branchial arches development. As the paralogue gene prep1.1 is more important in hindbrain patterning and neural crest chondrogenesis, we provide evidence of a functional specialization of prep genes in zebrafish head segmentation and morphogenesis.
Genes / Markers
Figures
Figure Gallery (12 images) / 2
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ia1TgTransgenic Insertion
    1 - 1 of 1
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    aldh1a2MO1-aldh1a2MRPHLNO
    pknox1.2MO1-pknox1.2MRPHLNO
    pknox1.2MO2-pknox1.2MRPHLNO
    tp53MO4-tp53MRPHLNO
    1 - 4 of 4
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    Fish
    Antibodies
    Orthology
    No data available
    Engineered Foreign Genes
    Marker Marker Type Name
    GFPEFGGFP
    1 - 1 of 1
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    Mapping
    No data available