FIGURE

Fig. 2

ID
ZDB-FIG-100628-35
Publication
Vaccari et al., 2010 - prep1.2 and aldh1a2 participate to a positive loop required for branchial arches development in zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Fig. 2

Morphological defects of prep1.2 morphant embryos. A: Schematic design of antisense morpholinos and prep1.2-GFP construct. The morpholinos are complementary to 25 bp in the 5′ region spanning the ATG (MO1-prep1.2) or to 25 bp in the 5′UTR region (MO2-prep1.2); MO2-prep1.2 is non-overlapping with the 5′UTR of the prep1.2-GFP mRNA injected and can only block endogenous prep1.2 mRNA. Two control morpholino oligos were designed. They are mutated in 5 (MO1-prep1.2-5MIS) or 6 (MO2-prep1.2-6MIS) positions. B, C: Each prep1.2 morpholino was co-injected with prep1.2-GFP mRNA to test its specificity; only MO1-prep1.2 blocks prep1.2-GFP translation in injected embryos (compare C with control morpholino in B) as revealed by the GFP signal in cell nuclei of the head region. D, E; day 5 larvae injected with MO1-prep1.2 display morphological defects: lack of branchial tissue, impaired jaw organization, absence of pectoral fins and pigmentation defects. F–I; Alcian blue staining of 5-day-old larvae shows that Mekel′s cartilage is normal while hyoid cartilage was misshaped and reduced in size. Morphant embryos also display a reduction of the third pharyngeal cartilage while the more posterior ones are completely absent. ba: branchial arches; pf: pectoral fin; cb: ceratobranchial; ch: ceratohyal; e: ethmoid plate; me: Meckel′s cartilage; tr: trabeculae cranii. D and E: Embryos are in dorsal view. F and G: Embryos are in ventral view. H and I: embryos are in lateral view.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 5

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 343(1-2), Vaccari, E., Deflorian, G., Bernardi, E., Pauls, S., Tiso, N., Bortolussi, M., and Argenton, F., prep1.2 and aldh1a2 participate to a positive loop required for branchial arches development in zebrafish, 94-103, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.