Excessive FoxD5 mRNA did not enable embryos to rescue the defects caused by tbx24-MO. (A–C) Lateral views of wild-type (WT) and embryos injected with different materials as indicated at 14 hpf. Compared to WT embryos (A), the arrays of the paired somites in tbx24 morphants (B) were lost. Injection of excessive FoxD5 mRNA could not rescue the loss of somite structure induced by the knockdown of tbx24 (C). WISH was performed to detect the expressions of myod, mespa and mespb in WT embryos and in embryos injected with different materials as indicated at 14 hpf. In WT embryos, the segmental expression of myod was detected (D). However, in the embryos injected with tbx24-MO, the segmental expression of myod was lost (E). The defective segmental pattern of myod in tbx24 morphants could not be rescued by injection of FoxD5 mRNA (F). WT embryos showed striped expressions of mespa (G) and mespb (K) in the anterior PSM. The segmental expressions of mespa (H; n = 89, 87%) and mespb (L; n = 68, 85%) were totally abolished in tbx24 morphants. In addition, the scattered expressions of mespa (I; n = 89, 13%) and mespb (M; n = 68, 15%) were also observed in the anterior PSM of tbx24 morphants. Injection of excessive FoxD5 mRNA did not enable embryos to rescue either the striped pattern or the expression levels of mespa (J; n = 47, 100%) and mespb (N; n = 53, 98%) in the tbx24 morphants.
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