PUBLICATION

FoxD5 mediates anterior-posterior polarity through upstream modulator Fgf signaling during zebrafish somitogenesis

Authors
Lee, H.C., Tseng, W.A., Lo, F.Y., Liu, T.M., and Tsai, H.J.
ID
ZDB-PUB-091023-32
Date
2009
Source
Developmental Biology   336(2): 232-245 (Journal)
Registered Authors
Tsai, Huai-Jen
Keywords
FoxD5, Fgf, Somitogenesis, Zebrafish
MeSH Terms
  • Animals
  • Base Sequence
  • Body Patterning*
  • DNA Primers
  • Fibroblast Growth Factors/metabolism*
  • Forkhead Transcription Factors/genetics
  • Forkhead Transcription Factors/physiology*
  • Gene Knockdown Techniques
  • Immunohistochemistry
  • Polymerase Chain Reaction
  • RNA, Messenger/genetics
  • Signal Transduction*
  • Somites/metabolism*
  • Zebrafish/embryology*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
PubMed
19818746 Full text @ Dev. Biol.
Abstract
The transcription factor FoxD5 is expressed in the paraxial mesoderm of zebrafish. However, the roles of FoxD5 in anterior pre-somitic mesoderm (PSM) during somitogenesis are unknown. We knocked down FoxD5 in embryos, which resulted in defects of the newly formed somites, including loss of the striped patterns of anterior-posterior polarity genes deltaC, notch2, notch3 and EphB2a, as well as the absence of mespa expression in S-I. Also, the expression of mespb exhibited a 'salt and pepper' pattern, indicating that FoxD5 is necessary for somite patterning in anterior PSM. Embryos were treated with SU5402, an Fgf receptor (FGFR) inhibitor, resulting in reduction of FoxD5 expression. This finding was consistent with results obtained from Tg(hsp70l:dnfgfr1-EGFP)pd1 embryos, whose dominant-negative form of FGFR1 was produced by heat-induction. Loss of FoxD5 expression was observed in the embryos injected with fgf3/fgf8-double-morpholinos (MOs). Excessive FoxD5 mRNA could rescue the defective expression levels of mespa and mespb in fgf3/fgf8 morphants, suggesting that Fgf signaling acts as an upstream modulator of FoxD5 during somitogenesis. We concluded that FoxD5 is required for maintaining anterior-posterior polarity within a somite and that the striped pattern of FoxD5 in anterior PSM is mainly regulated by Fgf. An Fgf-FoxD5-Mesps signaling network is therefore proposed.
Genes / Markers
Figures
Figure Gallery
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes