FIGURE

Fig. 4

ID

ZDB-FIG-091130-3

Publication

Hippe et al., 2009 - The interaction of nucleoside diphosphate kinase B with Gbetagamma dimers controls heterotrimeric G protein function

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Fig. 4

Loss of zebrafish Gβ₁ phenocopies NDPK B morphants. Cardiac phenotype of control morpholino-injected (MO-Ctrl) (A) and MO-Gβ₁/Gβ_1like double-injected (B) embryos at 72 hpf. Fractional shortening (FS) of the atrial and ventricular chamber of MO-Ctrl fishes (C) and Gβ₁/Gβ_1like morphants (D) at 48 and 72 hpf. Data are means ± SEM, n = 10–12. Histology sections of a MO-Ctrl (E) and Gβ₁/Gβ_1like knockdown (F) heart region stained with hematoxylin/eosin. A, atrium, V, ventricle, en, endocardial, my, myocardial layer. (G–J) Whole-mount immunofluorescence following staining with specific antibodies against atrial myosin heavy chain [S46, (G and I)] and entire heart tube myosin heavy chain [MF20, (H and J)] showing unaltered expression of structure proteins.

Expression Data

Gene:	myh6
Antibodies:	Ab-MF20 Ab-S46
Fish:	WT WT + MO1-gnb1a + MO1-gnb1b
Knockdown Reagents:	MO1-gnb1a MO1-gnb1b
Anatomical Terms:	atrium cardiac ventricle
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-gnb1a + MO1-gnb1b
Knockdown Reagents:	MO1-gnb1a MO1-gnb1b
Observed In:	heart heart contraction
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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