ZFIN ID: ZDB-FIG-090817-56
Sun et al., 2009 - Cardiac hypertrophy involves both myocyte hypertrophy and hyperplasia in anemic zebrafish. PLoS One   4(8):e6596 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage Range: High-pec to Adult
PHENOTYPE:
Fish:
Observed In:
Stage Range: Days 30-44 to Adult

Fig. 4 Myocyte hyperplasia contributes to all stages of cardiac remodeling in tr265/tr265.

(A–B′) Overlay of proliferating cell nuclear antigen (PCNA) antibody (red) with MEF2 antibody (green) in 10-μm sections of week-6 and -16 (A,B) sibling and (A′,B′) tr265/tr265 ventricles to show proliferating cardiomyocyte-lineage cells (yellow overlay; arrow) and proliferation of other cells (red overlay; arrowhead); bar = 50 μm. (C–C″′) Example of recently divided cells of the cardiomyocyte lineage (green) detected with BrdU antibody (red); bar = 10 μm. (D–D″′) Example of a proliferating mature cardiomyocyte (encircled) from a tr265/tr265 Tg(cmlc2:nuDsRed) mutant fish expressing DsRed, MEF2, and PCNA in its nucleus; bar = 10 μm. (E–E″′) Proliferating cardiomyocytes (encircled) derived from a cardiac progenitor cell in tr265/tr265 Tg(cmlc2:nuDsRed) transgenic fish containing unfolded DsRed in its cytoplasm and DsRed and PCNA in its nucleus. (F,G) Ratio of (F) PCNA+ MEF2+ cells over total MEF2+ cells or (G) BrdU+ MEF2+ cells over total MEF2+ cells in ventricles of week-6 and-16 sibling and tr265/tr265 fish, given as a percent; mean±SEM; * = P<0.05; n = number of fish examined.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 slc4a1atr265/tr265; f2Tg standard conditions High-pec cardiac muscle cell nucleus IFL
Adult cardiac muscle cell nucleus IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
slc4a1atr265/tr265 standard conditions Days 30-44 cardiac muscle cell hyperplastic, abnormal
Adult cardiac muscle cell hyperplastic, abnormal
slc4a1atr265/tr265; f2Tg standard conditions Days 30-44 cardiac muscle cell hyperplastic, abnormal
Adult cardiac muscle cell hyperplastic, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One