Chronic anemia induces cardiomegaly in tr265/tr265 zebrafish.
(A,A′) Pictures of the cardiomegaly in a week-16 (A′) tr265/tr265 and normal appearance of (A) a sibling; arrowhead points to the heart region. (B,B′) Dissected hearts of (B) the sibling and (B′) tr265/tr265; A, atrium, V, ventricle; bar = 1 mm. (C) Ventricle area to fish length (VA/BL) index used as a measurement of the cardiomegaly for week-4, -6, and -16 hearts. (D) Percent relative hemoglobin concentration at week 16. (E) Body length (in mm) at week 6 and 16. (F) Body mass (in grams) at week 6 and 16. (C–F) mean±SEM; * = P<0.05. n = number of fish examined.