Fig. S5

Buc-Specific Morpholinos Inhibit Translation of buc-GFP Fusion mRNA
Living embryos at high stage (∼3 hpf). Lateral view, animal to the top. Titration of the buc-specific morpholino (buc-MO) after co-injection of 2 μg/μl buc-GFP mRNA showed only background fluorescence of the vegetal yolk at 0.2 mM like in uninjected embryos, whereas embryos became fluorescent with 0.1 mM buc-MO. Control morpholinos showed no reduction of fluorescence, therefore in all injectionexperiments 0.2 mM morpholino together with 2 μg/μl buc mRNA was used. The results for buc-MO1 (5′-GACGAAGAAAATCAGCACAGGATAC-3′) and buc-MO2 (5′- TTTATTCCTTCCATTTCCTACGAGG-3′) or control-buc-MO1 (5′- GAgGAAcAAAATgAGCAgAGcATAC-3′) and control-buc-MO2 (5′- TTaATTgCTTCgATTTCgTACcAGG-3′) were simlar. Scale bar: 200 μm.