FIGURE

Fig. 2

ID

ZDB-FIG-090320-27

Publication

Stedman et al., 2009 - A functional interaction between Irx and Meis patterns the anterior hindbrain and activates krox20 expression in rhombomere 3

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Fig. 2

irx1b and irx7 are required to pattern the anterior hindbrain. (A–R) Embryos uninjected (A,C,E,G,I,K,M,O,Q) or coinjected with Mo1b and Mo7 (B, D, F, H, J, L, N, P, R) were stained by ISH with probes indicated on the left and colour-coded. Dotted lines in panels A and B indicate the AP length of the r1–r4 region. Arrowheads in panels B, F, H point to reduced expression domains of hoxb1a, cyp26c1 and cyp26b1, respectively. (S, T) Isl1-GFP transgenic embryos uninjected (S) or coinjected with Mo1b and Mo7 (T). The motoneurons of cranial nerves V (trigeminal, born in r2 and r3) and VII (facial, born in r4 and migrating into r6–r7) and the rhombomeres (r) are indicated. The dotted ovals show the position of the otic vesicle. All pictures are dorsal views of the hindbrain region of flat-mounted embryos, with rostral toward the left. The embryonic stage is indicated at the bottom-right of each picture.

Expression Data

Genes:	cyp26b1 cyp26c1 efnb2a egr2b fgf3 fgf8a fgfr3 GFP hoxa2b hoxb1a otx2b wnt1
Fish:	WT rw0Tg WT + MO2-irx1b + MO2-irx7 rw0Tg + MO2-irx1b + MO2-irx7
Knockdown Reagents:	MO2-irx1b MO2-irx7
Anatomical Terms:	cranial nerve V cranial nerve VII forebrain neural keel forebrain neural rod hindbrain neural keel midbrain hindbrain boundary neural keel midbrain neural keel midbrain neural rod presumptive rhombomere 2 presumptive rhombomere 3 presumptive rhombomere 4 presumptive rhombomere 5 rhombomere rhombomere 1 rhombomere 3 rhombomere 4 rhombomere 5
Stage Range:	1-4 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-irx1b + MO2-irx7 rw0Tg + MO2-irx1b + MO2-irx7
Knockdown Reagents:	MO2-irx1b MO2-irx7
Observed In:	cranial nerve V motor neuron cranial nerve VII motor neuron hindbrain development
Stage Range:	1-4 somites to Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 327(2), Stedman, A., Lecaudey, V., Havis, E., Anselme, I., Wassef, M., Gilardi-Hebenstreit, P., and Schneider-Maunoury, S., A functional interaction between Irx and Meis patterns the anterior hindbrain and activates krox20 expression in rhombomere 3, 566-577, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.