FIGURE

Fig. 7

ID

ZDB-FIG-090127-38

Publication

Sittaramane et al., 2009 - The cell adhesion molecule Tag1, transmembrane protein Stbm/Vangl2, and Lamininalpha1 exhibit genetic interactions during migration of facial branchiomotor neurons in zebrafish

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Fig. 7

Genetic interactions between tag1 and lama1. All panels show dorsal views of the hindbrain with anterior to the left. Tg(isl1:gfp) embryos (A–D) were fixed at 48 hpf, and processed for immunohistochemistry with zn5 antibody (red) to label dorsal commissural neurons and axons at rhombomere boundaries, and anti-GFP antibody (green) to label FBMNs (arrowheads). Non-transgenic embryos (E, F) were fixed at 30 hpf, and processed for tag1 in situ hybridization to label FBMNs (arrowheads). (A) FBMNs migrate normally in a control embryo. (B, C) Partial loss of FBMN migration in embryos injected with suboptimal dose of lama1 MO (B) or tag1 MO (C). (D) Complete loss of FBMN migration in an embryo injected with suboptimal doses of tag1 and lama1 MOs. (E) Greatly reduced FBMN migration in a bashful^uw1 homozygous (lama1 -/-) embryo. (H) Severe, nearly complete loss of FBMN migration in a putative bashful heterozygote injected with suboptimal dose of tag1 MO. Scale bar in D (75 μm for A–D); in F (75 μm for E, F).

Expression Data

Genes:	cntn2 GFP
Antibody:	zn-5
Fish:	lama1^uw1/uw1 rw0Tg + MO1-cntn2 rw0Tg + MO1-lama1 lama1^uw1/+ + MO1-cntn2 rw0Tg + MO1-cntn2 + MO1-lama1
Knockdown Reagents:	MO1-cntn2 MO1-lama1
Anatomical Terms:	brainstem and spinal white matter facial nerve motor nucleus motor neuron
Stage Range:	Prim-15 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lama1^uw1/uw1 rw0Tg + MO1-cntn2 rw0Tg + MO1-lama1 lama1^uw1/+ + MO1-cntn2 rw0Tg + MO1-cntn2 + MO1-lama1
Knockdown Reagents:	MO1-cntn2 MO1-lama1
Observed In:	facial nerve motor nucleus motor neuron neuron migration
Stage Range:	Prim-15 to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 325(2), Sittaramane, V., Sawant, A., Wolman, M.A., Maves, L., Halloran, M.C., and Chandrasekhar, A., The cell adhesion molecule Tag1, transmembrane protein Stbm/Vangl2, and Lamininalpha1 exhibit genetic interactions during migration of facial branchiomotor neurons in zebrafish, 363-373, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.