PUBLICATION

The cell adhesion molecule Tag1, transmembrane protein Stbm/Vangl2, and Lamininalpha1 exhibit genetic interactions during migration of facial branchiomotor neurons in zebrafish

Authors
Sittaramane, V., Sawant, A., Wolman, M.A., Maves, L., Halloran, M.C., and Chandrasekhar, A.
ID
ZDB-PUB-081121-15
Date
2009
Source
Developmental Biology   325(2): 363-373 (Journal)
Registered Authors
Chandrasekhar, Anand, Halloran, Mary, Maves, Lisa, Sittaramane, Vinoth, Wolman, Marc
Keywords
Hindbrain, Motor neuron, Branchiomotor, Neuronal migration, Time-lapse imaging, Cell adhesion molecule, Tag1, Van gogh-like, Laminin, Genetic interaction
MeSH Terms
  • Animals
  • Cell Adhesion
  • Cell Adhesion Molecules, Neuronal/physiology*
  • Cell Movement
  • Contactin 2
  • Laminin/physiology*
  • Membrane Proteins/physiology*
  • Motor Neurons/physiology*
  • Rhombencephalon/embryology*
  • Rhombencephalon/physiology
  • Signal Transduction
  • Zebrafish/embryology*
  • Zebrafish/physiology
  • Zebrafish Proteins/physiology*
PubMed
19013446 Full text @ Dev. Biol.
Abstract
Interactions between a neuron and its environment play a major role in neuronal migration. We show here that the cell adhesion molecule Transient Axonal Glycoprotein (Tag1) is necessary for the migration of the facial branchiomotor neurons (FBMNs) in the zebrafish hindbrain. In tag1 morphant embryos, FBMN migration is specifically blocked, with no effect on organization or patterning of other hindbrain neurons. Furthermore, using suboptimal morpholino doses and genetic mutants, we found that tag1, lamininalpha1 (lama1) and stbm, which encodes a transmembrane protein Vangl2, exhibit pairwise genetic interactions for FBMN migration. Using time-lapse analyses, we found that FBMNs are affected similarly in all three single morphant embryos, with an inability to extend protrusions in a specific direction, and resulting in the failure of caudal migration. These data suggest that tag1, lama1 and vangl2 participate in a common mechanism that integrates signaling between the FBMN and its environment to regulate migration.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes