Fig. S3 III

III. A–D. Mosaic reporter expression in the skin epithelia. A–D. The krt8:LexPR-LexOP:EGFP driver construct contains a promoter of the keratin8 (krt8) gene that drives expression of LexPR transactivator in the single layer of epithelial skin cells of the same type. Shown are four F₂ fish from the same parents. Although most epithelial cells express both reporters, there is visible variation of intensity between cells. There are also patches of cells that express very weak level of both reporters (large arrow), that may reflect positional effects at the driver insertion site or silencing of the LexPR gene, the difference in drug permeability among the cells of the same type or other difference between these cells (age, stress, etc) at the time of induction. A. Assembled image of the whole fish produced by stitching several overlapped exposures.[GR7]