FIGURE

Fig. 9

ID

ZDB-FIG-080730-12

Publication

Sharma et al., 2008 - Regionalized calcium signaling in zebrafish fertilization

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Fig. 9

Localization of Fyn in the zebrafish oocyte. Oocytes were fixed before (A) or at different times after fertilization: 2.5 mpi (B,D), 45 mpi (C). The fixed oocytes were then processed for immunofluorescence. Oocytes were labeled with the anti-Fyn3 antibody (A,B,C) at a concentration of 0.5 μg/ml or with Fyn3 antibody + the synthetic peptide antigen (1 mM) as a control (D). Bound antibody was detected with goat anti-rabbit IgG conjugated with Alexa Fluor-488. Prior to and immediately after fertilization, the distribution of Fyn kinase differs from that of the IP3 receptor in that it is concentrated at the animal pole even before fertilization (A) and at the site of sperm fusion (white arrow, B). By 45 mpi (C), Fyn was localized over the blastodisc cortex and was usually concentrated within a smaller region of the blastodisc possibly indicating a region of intense signaling.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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