FIGURE

Fig. 4

ID

ZDB-FIG-080529-68

Publication

Zhu et al., 2003 - Cloning, expression, and characterization of a membrane progestin receptor and evidence it is an intermediary in meiotic maturation of fish oocytes

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Fig. 4

Inhibition of cAMP production (a) and activation of MAP kinase signaling pathway (b and c) in response to progestin hormones in MDA-MB-231 cells stably transfected with putative mPR cDNA (means ± SEM, n = 4; *, P < 0.05). (a) Cells were preexposed to activated pertussis toxin (PTX) or inactive pertussis toxin (inPTX) for 30 min at 37°C before incubation with 20β-S or progesterone for 5 min. (b) MAP kinase activity (shown as increase in phospho-Erk1/2) in cells transfected with vector containing mPR insert (white bar), controls containing reversed insert (shaded bar) or vector alone (black bar) after a 5-min stimulation with 1 μM 20β-S or progesterone (P₄). C, untreated control; EGF, human epidermal growth factor (1 μM, positive control). (c) Time- and dose-dependent activation of MAP kinase (gel loading: 20 μg per lane) in transfected cells treated with 20β-S, P₄, estradiol-17β (E₂)_, or testosterone (T).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA