FIGURE

Fig. 6

ID
ZDB-FIG-080328-15
Publication
Bit-Avragim et al., 2008 - Nuclear localization of the zebrafish tight junction protein nagie oko
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Fig. 6

nok and aPKCi are not sufficient to tether each other within the nucleus. Images are reconstructions of confocal Z-stack sections imaged on late gastrula stage whole mounts. Myc and aPKC stainings are false-colored. A,B: Embryos expressing high levels of HisMyc-tagged nokΔNES were counterstained with anti aPKC. A,A′: Within DL cells, high levels of nuclear HisMyc-tagged nokΔNES are present whereas aPKC localizes to outer cell membranes and no increased levels of nuclear aPKC are detectable (A“). B,B′: Within epithelial EVL cells, HisMyc-tagged nokΔNES is at outer cell membranes and within the nucleus. B”: aPKC is predominantly associated with outer cell membranes. C: Embryos overexpressing HisMyc-tagged nokwt (green) and non-tagged aPKCiKD (red), whereas nokwt is absent from the nucleus (C′), aPKCiKD is nuclear. All images are apical views onto the tissue.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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