FIGURE

Fig. 5

ID

ZDB-FIG-080328-14

Publication

Bit-Avragim et al., 2008 - Nuclear localization of the zebrafish tight junction protein nagie oko

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Fig. 5

aPKC kinase catalytic activity prevents nuclear accumulation. Images are reconstructions of confocal Z-stack sections imaged on late gastrula stage whole mounts. Anti aPKC (A,B,D,E) and anti-Myc (C) stainings are false-colored. A: Endogenous aPKC localization in EVL cells is mostly at the outer cell membrane whereas (B) weak levels of wt protein are also detectable throughout the cytoplasm and nuclei of DL cells. C: HisMyc-tagged aPKCi^D57A within EVL cells localizes to the cytoplasm and is complete absent from outer cell membranes or nuclei. D: aPKCi^KD strongly localizes to the nuclei of EVL and (E) DL cells. All images are apical views onto the tissue.

Expression Data

Gene:	prkci
Fish:	AB
Anatomical Terms:	DEL EVL
Stage:	75%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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