FIGURE

Fig. 5

ID

ZDB-FIG-080325-65

Publication

Wilkins et al., 2008 - Mtx2 directs zebrafish morphogenetic movements during epiboly by regulating microfilament formation

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Fig. 5

Normal endoderm and shield specification in mtx2 morphants. Whole-mount in situ hybridization for faust/gata5 (A, D) and mixer (B, E) shows endoderm specification in mtx2 MO injected embryos (D, E). The blastoderm margin appears a little irregular in mtx2 MO injected embryos (D–F). Goosecoid (gsc) is expressed in the shield of mtx2 MO injected embryos (F) but is reduced when compared with std MO injected embryos (C). All embryos are at shield stage. Panels A and D are animal pole views; panels B and E are lateral or oblique views; panels C and F are dorsal midline views. AP = animal pole, VP = vegetal pole. Scale bar = 200 μm in panel A (applies to panels A–F).

Expression Data

Genes:	gata5 gsc mixl1
Fish:	WT WT + MO1-mxtx2
Knockdown Reagent:	MO1-mxtx2
Anatomical Terms:	presumptive endoderm shield
Stage:	Shield

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-mxtx2
Knockdown Reagent:	MO1-mxtx2
Observed In:	margin shield
Stage:	Shield

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 314(1), Wilkins, S.J., Yoong, S., Verkade, H., Mizoguchi, T., Plowman, S.J., Hancock, J.F., Kikuchi, Y., Heath, J.K., and Perkins, A.C., Mtx2 directs zebrafish morphogenetic movements during epiboly by regulating microfilament formation, 12-22, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.