ZFIN ID: ZDB-FIG-070129-18
Jeong et al., 2006 - Neurogenin1 is a determinant of zebrafish basal forebrain dopaminergic neurons and is regulated by the conserved zinc finger protein Tof/Fezl. Proceedings of the National Academy of Sciences of the United States of America   103(13):5143-5148 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-5

Fig. 1

ngn1 is expressed in DA progenitors. All images are lateral views of anterior brain regions. Anterior is to the left, and dorsal is up. (A and B) ngn1 expression at 14- and 20-somite stages, respectively (arrows point to several clusters in the ventral forebrain). (C) A 28-hpf embryo showing ngn1 expression (purple) in close proximity to TH+ DA neurons (red). (DF) Confocal images of 28 hpf ngn1-GFP transgenic embryos immunostained with GFP antibody (green, D), TH antibody (red, E), and the merged image (F), showing that GFP is detected in TH+ DA neurons. (GI) High-magnification views of DF. (JL) Confocal images of 28-hpf ngn1-GFP transgenic embryos immunostained with GFP antibody (green, J), 5HT antibody (red, K), and the merged image (L), showing that GFP is not detected in 5HT neurons. (MO) High-magnification views of JL. di, diencephalon, t, telencephalon. (Scale bars, 64 μm in A and B, 60 μm in CF and JL, and 3 μm in GI and MO.)

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
neurog1 WT standard conditions 14-19 somites forebrain ISH
20-25 somites forebrain ISH
Prim-5 forebrain ISH
th sb1Tg standard conditions Prim-5 dopaminergic neuron IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA